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KMID : 0604520050310040323
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Journal of the Society of Cosmetic Scientists of Korea
2005 Volume.31 No. 4 p.323 ~ p.327
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Antioxidant Constituents from Melothria heterophylla; Regulation of Matrix Metalloproteinase-1 Expression in Ultraviolet A-irradiated Human Dermal Fibroblasts
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Cho Young-Ho
Kim Jin-Hee
Sim Gwan-Sub
Lee Beom-Cheon
Pyo Hyeong-Bae
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Abstract
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Although many studies have been performed to elucidate the molecular consequence of ultraviolet irradiation on an aging, little is known about the effect of natural products. Matrix metalloproteinases (MMPs) we known to play an important role in (a) photoaging. Hete we investigated the effect of 1,2,4,6-tetra-O-galloyl-{beta}-{_D}-gluco- pyranose (1) and 3,4,5-trihydroxybenzoic acid (2) on the expression of MMP-1 in UVA-irradiated human skin fibroblasts (products), (on the) activity of MMP-1, and (on the) scavenging activities of free radicals. Compounds 1 and 2 were isolated from Melothria heterophylla (Cucurbitaceae). These compounds were found to scavenge free radicals and reactive oxygen species (ROS) and were measured to have the SC_{50} values of 3.9{mu}M;and;13.3{mu}M against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 4.3{mu}M;and; 4.0{mu}M against superoxide radicals in the xanthine/xanthine oxidase system, respectively. Compounds 1 and 2 showed a dose-dependent inhibitory effect on the e):pression and activity of MMP-1 in the UVA-irradiated human skin fibroblasts. Therefore, we concluded that compounds 1 and 2 significantly inhibited MMP-1 expression at the protein level. Also, these compounds were determined to have a potent antioxidant activity. From these results, we suggest that these compounds nay be used as (a) new anti-aging agents for the photo-damaged skin.
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KEYWORD
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Melothria heterophylla, antioxidants, UVA irradiation, MMP-1, skin aging
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